Hydration of Hyaluronan : Effects on Structural and Thermodynamic Properties

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Hydration of Hyaluronan : Effects on Structural and Thermodynamic Properties


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Publication Article, peer reviewed scientific
Title Hydration of Hyaluronan : Effects on Structural and Thermodynamic Properties
Author Albèr, Cathrine ; Engblom, Johan ; Falkman, Peter ; Kocherbitov, Vitaly
Research Centre Biofilms - Research Center for Biointerfaces
Date 2015
English abstract
Hyaluronan (HA) is a frequently occurring biopolymer with a large variety of functions in nature. During the past 60 years, there have been numerous reports on structural and dynamic behavior of HA in water. Nevertheless, studies covering a wider concentration range are still lacking. In this work, we use isothermal scanning sorption calorimetry for the first time to investigate hydration-induced transitions in HA (sodium hyaluronate, 17 kDa). From this method, we obtain the sorption isotherm and the enthalpy and the entropy of hydration. Thermotropic events are evaluated by differential scanning calorimetry (DSC), and structure analysis is performed with X-ray scattering (SWAXS) and light and scanning electron microscopy. During isothermal hydration, HA exhibits a glass transition, followed by crystallization and subsequent dissolution of HA crystals and formation of a one-phase solution. Structural analysis reveals that the crystal may be indexed on an orthorhombic unit cell with space group P212121. Crystallization of HA was found to occur either through endothermic or exothermic processes, depending on the temperature and water content. We propose a mechanism of crystallization that explains this phenomenon based on the interplay between the hydrophobic effect and strengthening of hydrogen bonds during formation of crystals. The combined results were used to construct a binary phase diagram for the HA–water system.
DOI https://doi.org/10.1021/jp511542e (link to publisher's fulltext.)
Publisher American Chemical Society
Host/Issue The Journal of Physical Chemistry B;11
Volume 119
ISSN 1520-6106
Pages 4211-4219
Language eng (iso)
Subject Hyaluronic acid
Sorption calorimetry
Phase transitions
Phase diagram
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/19835 Permalink to this page

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