Osteogenic potential of human adipose-derived stromal cells on 3-dimensional mesoporous TiO2 coating with magnesium impregnation

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Osteogenic potential of human adipose-derived stromal cells on 3-dimensional mesoporous TiO2 coating with magnesium impregnation

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Publication Article, peer reviewed scientific
Title Osteogenic potential of human adipose-derived stromal cells on 3-dimensional mesoporous TiO2 coating with magnesium impregnation
Author(s) Cecchinato, Francesca ; Karlsson, Johan ; Ferroni, Letizia ; Gardin, Chiara ; Galli, Silvia ; Wennerberg, Ann ; Zavan, Barbara ; Andersson, Martin ; Jimbo, Ryo
Date 2015
English abstract
The aim of this study was to evaluate the osteogenic response of human adipose-derived stromal cells (ADScs) to mesoporous titania (TiO2) coatings produced with evaporation-induced self-assembly method (EISA) and loaded with magnesium. Our emphasis with the magnesium release functionality was to modulate progenitor cell osteogenic differentiation under standard culture conditions. Osteogenic properties of the coatings were assessed for stromal cells by means of scanning electron microscopy (SEM) imaging, colorimetric mitochondrial viability assay (MTT), colorimetric alkaline phosphates activity (ALP) assay and real time RT-polymerase chain reaction (PCR). Using atomic force microscopy (AFM) it was shown that the surface expansion area (Sdr) was strongly enhanced by the presence of magnesium. From MTT results it was shown that ADSc viability was significantly increased on mesoporous surfaces compared to the non-porous one at a longer cell culture time. However, no differences were observed between the magnesium impregnated and non-impregnated surfaces. The alkaline phosphatase activity confirmed that ADSc started to differentiate into the osteogenic phenotype after 2weeks of culturing. The gene expression profile at 2weeks of cell growth showed that such coatings were capable to incorporate specific osteogenic markers inside their interconnected nano-pores and, at 3weeks, ADSc differentiated into osteoblasts. Interestingly, magnesium significantly promoted the osteopontin gene expression, which is an essential gene for the early biomaterial-cell osteogenic interaction.
DOI http://dx.doi.org/10.1016/j.msec.2015.03.026 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Material Science and Engineering C;
Volume 52
ISSN 1873-0191
Pages 225-234
Language eng (iso)
Subject(s) 3-D nanostructure
Magnesium
Osteogenesis
Osteogenic markers
Osteopontin
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/19991 (link to this page)

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