Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles

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Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles

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Publication Article, peer reviewed scientific
Title Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles
Author Barauskas, Justas ; Anderberg, Hanna ; Svendsen, Allan ; Nylander, Tommy
Date 2016
English abstract
In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between the catalytic activity, measured by pH-stat titration, and the change in morphology and nano-structure determined by cryogenic transmission electron microscopy and synchrotron small angle X-ray diffraction. The initial lipase catalyzed LCNP hydrolysis rate is approximately 25% higher for large 350 nm nanoparticles compared to the small 190 nm particles, which is attributed to the increased number of structural defects on the particle surface. At pH 8.0 and 8.4 bicontinuous Im3m cubic LCNPs transform into "sponge"-like assemblies and disordered multilamellar onion-like structures upon exposure to lipase. At pH 6.5 and 7.5 lipolysis induced phase transitions of the inner core of the particles, following the sequence Im3m cubic -> reversed hexagonal -> reversed micellar Fd3m cubic -> reversed micelles. These transitions to the liquid crystalline phases with higher negative curvature of the lipid/water interface were found to trigger protonation of the oleic acid produced during lipase catalyzed reaction. The increase curvature of the reversed discrete micellar cubic phase was suggested to cause an increase in the oleic acid pK(a) to a larger value observed by pH-stat titration. (C) 2015 Elsevier B.V. All rights reserved.
DOI https://doi.org/10.1016/j.colsurfb.2015.04.052 (link to publisher's fulltext.)
Publisher Elsevier
Host/Issue COLLOIDS AND SURFACES B-BIOINTERFACES;
Volume 137
ISSN 0927-7765
Pages 50-59
Language eng (iso)
Subject Lipase
Cubic phase
Lipid liquid crystalline nanoparticles
Hydrolysis;
X-ray diffraction
pH-stat titration
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/21927 Permalink to this page
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