Different expression levels of glycans on leukemic cells-a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

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Different expression levels of glycans on leukemic cells-a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

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Publication Article, peer reviewed scientific
Title Different expression levels of glycans on leukemic cells-a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid
Author El-Schich, Zahra ; Abdullah, Mohammad ; Shinde, Sudhirkumar ; Dizeyi, Nishtman ; Rosén, Anders ; Sellergren, Börje ; Gjörloff Wingren, Anette
Research Centre Biofilms - Research Center for Biointerfaces
Date 2016
English abstract
Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell lines, conveniently analyzed by flow cytometry and fluorescence microscopy. SA expression was detected in four cell lines at different levels, and the SA expression were verified with lectin-FITC. These results show that SA-MIP can be used as a plastic antibody for detection of SA using both flow cytometry and fluorescence microscopy. We suggest that SA-MIP can be used for screening of different tumor cells of various stages, including CLL cells.
DOI https://doi.org/10.1007/s13277-016-5280-y (link to publisher's fulltext.)
Link https://www.ncbi.nlm.nih.gov/pmc/articles/PMC50970... (external link to publication)
Publisher Springer
Host/Issue Tumor Biology;37
Volume 10
ISSN 1423-0380
Pages 13763-13768
Language eng (iso)
Subject Chronic lymphocytic leukemia
Lectin
Molecular imprinting polymers
Sialic acid
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/21976 Permalink to this page
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