Reducing dental plaque formation and caries development : A review of current methods and implications for novel pharmaceuticals.

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Reducing dental plaque formation and caries development : A review of current methods and implications for novel pharmaceuticals.

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Publication Article, peer reviewed scientific
Title Reducing dental plaque formation and caries development : A review of current methods and implications for novel pharmaceuticals.
Author Kalesinskas, Povilas ; Kačergius, Tomas ; Ambrozaitis, Arvydas ; Pečiulienė, Vytautė ; Ericson, Dan
Date 2014
English abstract
Dental caries remains one of the most prevalent human diseases due to uncontrolled increase of dietary sucrose consumption in modern society. Sucrose is metabolized by Streptococcus mutans and Streptococcus sobrinus to acids causing tooth decay. These streptococci produce glucosyltransferases (Gtfs) for synthesis of sticky glucan polymers from sucrose, which is important for biofilm formation on teeth. In order to reduce dental biofilm build-up and oral diseases it causes, one preventive measure could be blocking of Gtf synthesis. Therefore, aim of this study was to test antisense phosphorothioate oligodeoxyribonucleotide (PS-ODN) targeting simultaneously S. mutans gtfB, gtfC and S. sobrinus gtfI mRNAs in order to inhibit biofilm formation in vitro. Materials and methods. Mixed culture of S. mutans and S. sobrinus bacteria were grown anaerobically on glass slides inserted vertically in 24-well cell culture plates containing Todd Hewitt broth with sucrose and sterile saliva under exposure to antisense or missense PS-ODNs at final concentration of 10 microM. Untreated bacteria served as controls. After 24 h of incubation, glass slides were removed, air-dried and further used for quantitative evaluation of streptococci biofilm applying optical profilometry technique. Results. It was found that antisense PS-ODN significantly reduced biofilm surface roughness and thickness of mixed S. mutans and S. sobrinus culture suppressing the biofilm development by 1.5-fold overall in comparison to untreated and missense PS-ODN-treated bacteria. Conclusions. Data demonstrate that antisense PS-ODN considerably attenuate streptococci-induced biofilm build-up on glass slides, and might therefore significantly inhibit dental biofilm formation through simultaneous inactivation of bacterial gtf mRNAs.
Link http://sbdmj.lsmuni.lt/142/142-02.pdf .Icon
Publisher Odontologijos Studija
Host/Issue Stomatologija;2
Volume 16
ISSN 1392-6470
Pages 44-52
Language eng (iso)
Subject dental caries
Streptococcus sobrinus,
Streptococcus mutans
biofilm
glucosyltransferase
antisense oligonucleotide
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/22000 Permalink to this page
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