Holography : The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

DSpace Repository

Holography : The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

Details

Files for download
Book chapter
Overview of item record
Publication BookChapter
Title Holography : The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics
Author El-Schich, Zahra ; Kamlund, Sofia ; Janicke, Birgit ; Alm, Kersti ; Gjörloff Wingren, Anette
Editor Naydenova, Izabela ; Nazarova, Dimana ; Babeva, Tsvetanka
Date 2017
English abstract
Digital holographic (DH) microscopy is a digital high-resolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells. The unique measurable parameters are the cell number, cell area, thickness, and volume, which can be coupled to proliferation, migration, cell cycle analysis, viability, and cell death. The technique is cell friendly, fast and simple to use and has unique imaging capabilities for time-lapse investigations on both the single cell and the cell-population levels. The interest for analyzing specifically cell volume changes with DH microscopy, resulting from cytotoxic treatments, drug response, or apoptosis events has recently increased in popularity. We and others have used DH microscopy showing that the technique has the sensitivity to distinguish between different cells and treatments. Recently, DH microscopy has been used for cellular diagnosis in the clinic, providing support for using the concept of DH, e.g., screening of malaria infection of red blood cells (RBC), cervix cancer screening, and sperm quality. Because of its quick and label-free sample handling, DH microscopy will be an important tool in the future for personalized medicine investigations, determining the optimal therapeutic concentration for both different cancer types and individual treatments.
DOI https://doi.org/10.5772/66042 (link to publisher's fulltext.)
Link https://www.intechopen.com/books/holographic-materials-and-optical-systems/holography-the-usefulness-of-digital-holographic-microscopy-for-clinical-diagnostics .Icon
Publisher Intech
Host/Issue Holographic Materials and Optical Systems
ISBN 978-953-51-3038-3
Pages 319-333
Language eng (iso)
Subject cell death
cell volume
digital holographic microscopy
individual treatment
Technology
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/22451 Permalink to this page
Facebook

This item appears in the following Collection(s)

Details

Search


Browse

My Account

Statistics