Controlled release of Clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects

DSpace Repository

Controlled release of Clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects

Details

Files for download Overview of item record
Publication Article, peer reviewed scientific
Title Controlled release of Clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects
Author Alenezi, Ali ; Naito, Yoshihito ; Terukina, Takayuki ; Prananingrum, Widyasri ; Jinno, Yohei ; Tagami, Tatsuaki ; Ozeki, Tetsuya ; Galli, Silvia ; Jimbo, Ryo
Research Centre Biofilms - Research Center for Biointerfaces
Date 2017
English abstract
Purpose: To evaluate the controlled release effect of Clarithromycin loaded in PLGA microspheres in a rabbit calvaria defect model. Methods: Clarithromycin-loaded PLGA microspheres (MSPs) were formulated by modified O/W single emulsion/solvent evaporation method. After characterization, in vivo animal experiment was conducted. Four critical size bone defects were created in the calvaria of New Zealand White rabbits (n=21, n=7/time point). The bone defects were randomly designated to 4 groups: Group 1: No augmentation (sham), Group 2: beta-Tricalcium phosphate (β-TCP), Group 3: beta-Tricalcium phosphate (β-TCP) with 0.12 µg clarithromycin, and Group 4: beta-Tricalcium phosphate (β-TCP) with 6.12 µg PLGA microspheres (loaded with 0.12 µg clarithromycin). After 2, 4 and 12 weeks of healing, the levels of bone regeneration were evaluated using micro- computed tomography and histology. Results: The average size of the PLGA microspheres was 26.38 μm that showed 94% encapsulation efficacy with clarithromycin. Clarithromycin release from PLGA microspheres revealed sustained release for around 4 weeks with approximately 50% release of clarithromycin during the first week. In the histological analysis, new bone formation was evident at 2 and 4 weeks of healing in all groups and bone formation increased as a function of healing time in vivo. At 12 weeks, Group 4 showed significantly higher amount of newly formed bone compared to Group 1 (p=0,002). Moreover, during the micro CT exam, Group 4 expressed significantly higher bone formation compared to Group 1 at all time points tested (p=0.00, 0.014, and 0.002 in 2, 4, and 12 weeks, respectively). Conclusions: PLGA microspheres demonstrated initial burst release of clarithromycin followed by a sustained release profile. The in vivo findings showed that β-TCP with clarithromycin-loaded microspheres can enhance bone formation in bone defects.
DOI https://doi.org/10.1002/jbm.b.33844 (link to publisher's fulltext.)
Link http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.3... (external link to publication)
Publisher Wiley
Host/Issue Journal of Biomedical Materials Research Part B : Applied Biomaterials;00
Volume 00B
ISSN 1552-4981
Pages 19
Language eng (iso)
Subject microspheres
drug delivery system
sustained release
clarithromycin
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/22685 Permalink to this page
Facebook

This item appears in the following Collection(s)

Details

Search


Browse

My Account

Statistics