Diffusion through Pig Gastric Mucin: Effect of Relative Humidity

DSpace Repository

Diffusion through Pig Gastric Mucin: Effect of Relative Humidity

Details

Files for download Overview of item record
Publication Article, peer reviewed scientific
Title Diffusion through Pig Gastric Mucin: Effect of Relative Humidity
Author Runnsjö, Anna ; Dabkowska, Aleksandra P ; Sparr, Emma ; Kocherbitov, Vitaly ; Arnebrant, Thomas ; Engblom, Johan
Research Centre Biofilms - Research Center for Biointerfaces
Date 2016
English abstract
Mucus covers the epithelium found in all intestinal tracts, where it serves as an important protecting barrier, and pharmaceutical drugs administrated by the oral, rectal, vaginal, ocular, or nasal route need to penetrate the mucus in order to reach their targets. Furthermore, the diffusion in mucus as well as the viscosity of mucus in the eyes, nose and throat can change depending on the relative humidity of the surrounding air. In this study we have investigated how diffusion through gels of mucin, the main protein in mucus, is affected by changes in ambient relative humidity (i.e. water activity). Already a small decrease in water activity was found to give rise to a significant decrease in penetration rate through the mucin gel of the antibacterial drug metronidazole. We also show that a decrease in water activity leads to decreased diffusion rate in the mucin gel for the fluorophore fluorescein. This study shows that it is possible to alter transport rates of molecules through mucus by changing the water activity in the gel. It furthermore illustrates the importance of considering effects of the water activity in the mucosa during development of potential pharmaceuticals.
DOI https://doi.org/10.1371/journal.pone.0157596 (link to publisher's fulltext.)
Link http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157596 .Icon
Publisher Public Library of Science
Host/Issue PLOS ONE;6
Volume 11
ISSN 1932-6203
Pages e0157596
Language eng (iso)
Subject Multidisciplinary Sciences
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/23043 Permalink to this page
Facebook

This item appears in the following Collection(s)

Details

Search


Browse

My Account

Statistics