Monoclonal antibody capture from cell culture supernatants using epitope imprinted macroporous membranes

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Monoclonal antibody capture from cell culture supernatants using epitope imprinted macroporous membranes

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Publication Article, peer reviewed scientific
Title Monoclonal antibody capture from cell culture supernatants using epitope imprinted macroporous membranes
Author Schwark, Sebastian ; Sun, Wei ; Stute, Jörg ; Lütkemeyer, Dirk ; Ulbricht, Mathias ; Sellergren, Börje
Date 2016
English abstract
Epitope-imprinted membranes targeting the C-terminal fragment of the immunoglobuline G (IgG) heavy chain was developed and used for the purification of a commercial monoclonal antibody. The membranes exhibited strongly enhanced IgG affinity when compared with non-imprinted or IgG imprinted membranes reflected in binding selectivities in a protein mixture (IgG/HSA 1 : 10 w/w) of up to 40, and the elution of 95 to 100% pure IgG after washing. The dynamic binding capacity amounted to 3.9 mg mL(-1) membrane volume with minor loss in performance upon repeated cleaning with alkali. The depletion of host cell proteins from a cell culture broth after production of anti-IL8 antibody using the best performing imprinted membrane under low-salt conditions reached 88% (0.7-1.2 log units) implying an effective removal of impurities from the cell culture supernatant.
DOI https://doi.org/10.1039/c6ra06632a (link to publisher's fulltext.)
Link http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA06632A#!divAbstract .Icon
Publisher Royal Society of Chemistry
Host/Issue RSC Advances;58
Volume 6
ISSN 2046-2069
Pages 53162-53169
Language eng (iso)
Subject Chemistry, Multidisciplinary
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/23063 Permalink to this page
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