Bactericidal effect of photocatalytically-active nanostructured TiO2 surfaces on biofilms of the early oral colonizer, Streptococcus oralis

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Bactericidal effect of photocatalytically-active nanostructured TiO2 surfaces on biofilms of the early oral colonizer, Streptococcus oralis

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Publication Article, peer reviewed scientific
Title Bactericidal effect of photocatalytically-active nanostructured TiO2 surfaces on biofilms of the early oral colonizer, Streptococcus oralis
Author Westas, Emma ; Hayashi, Mariko ; Cecchinato, Francesca ; Wennerberg, Ann ; Andersson, Martin ; Jimbo, Ryo ; Davies, Julia
Date 2017
English abstract
This study evaluated the photocatalytic bactericidal effect of nanostructured anatase-rich titanium dioxide (TiO2 ) on microbial biofilms. Commercially pure titanium discs were spin-coated with photocatalytic TiO2 nanoparticles (P25). Uncoated discs were used as control (CTRL). Half of the CTRL and half of the P25-coated surfaces were coated with purified saliva (SAL) to give four different groups (CTRL, CTRL + SAL, P25 and P25 + SAL). Streptococcus oralis were allowed to form biofilms on the discs for 18 h and non-adherent cells were rinsed off. Bacterial viability was assessed at time 0 with Live/Dead BacLight staining and epifluorescence microscopy. The remaining discs were divided into a non-UV group and UVA-irradiated (+UV) group (irradiation time, 6 or 24 h). Thereafter, viability was assessed as above. Viability at time 0 was high and no dead cells were seen on any of the surfaces, even after 24 h, in the absence of UVA. However, after 24 h of exposure, the proportion of viable cells was reduced by 40% on the P25 discs compared to 0 and 6 h, and this effect was enhanced with a salivary pellicle. Members of mixed species biofilms differ in their susceptibility to the bactericidal effect of the surfaces tested and further investigations are needed to optimize the conditions.
DOI https://doi.org/10.1002/jbm.a.36086 (link to publisher's fulltext.)
Publisher Wiley
Host/Issue Journal of Biomedical Materials Research Part A;8
Volume 105
ISSN 1552-4965
Pages 2321-2328
Language eng (iso)
Subject bactericidal effect;
biofilm
photocatalyst
titanium implant
ultraviolet A
Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/23303 Permalink to this page
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