Human palatal saliva: Adsorption behaviour and the role of low-molecular weight proteins

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Human palatal saliva: Adsorption behaviour and the role of low-molecular weight proteins

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Publication Article, peer reviewed scientific
Title Human palatal saliva: Adsorption behaviour and the role of low-molecular weight proteins
Author(s) Svendsen, Ida ; Arnebrant, Thomas ; Lindh, Liselott
Date 2004
English abstract
In situ ellipsometry was employed to study adsorption from human palatal saliva (HPalS) in terms of dependence on surface wettability and saliva concentration (<or=1%). Adsorbed amounts, kinetics, and elutability with buffer and sodium dodecyl sulphate (SDS) were determined. The low-molecular weight protein content of bulk HPalS was also investigated using two-dimensional gel electrophoresis, and this revealed the presence of a large group of proteins<100 kDa in size. Adsorption to pure (hydrophilic) and methylated (hydrophobized) silica surfaces revealed that the total adsorbed amounts were greater on hydrophobized silica. Below concentrations of 0.5 and 0.25% saliva, adsorption was concentration dependent on hydrophobized and hydrophilic surfaces, respectively. The initial adsorption (<or=30 min) was faster on hydrophobized surfaces. Addition of SDS removed more material than buffer rinsing on both surfaces. Analysis of the adsorption kinetics indicated that the presence of low-molecular weight proteins plays a role in adsorption from HPalS.
DOI http://dx.doi.org/10.1080/08927010400028991 (link to publisher's fulltext)
Publisher Taylor & Francis
Host/Issue Biofouling;6
Volume 20
ISSN 0892-7014
Pages 269-277
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Surface and colloid chemistry Research Subject
Handle http://hdl.handle.net/2043/2686 (link to this page)

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