Enhanced DNA-dependent Protein Kinase Activity in Sjögren's Syndrome B Cells

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Enhanced DNA-dependent Protein Kinase Activity in Sjögren's Syndrome B Cells

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Publication Article, other scientific
Title Enhanced DNA-dependent Protein Kinase Activity in Sjögren's Syndrome B Cells
Author(s) Henriksson, Gunnel ; Sallmyr, Annahita ; Du, L ; Larsson, Åke ; Manthorpe, Rolf ; Bredberg, Anders
Date 2004
English abstract
Objective. To examine the stress response, including the role of DNA-dependent protein kinase (DNA-PK), in B cells from Sjögren's syndrome (SS) patients. Methods. B-cell lines were exposed to gamma radiation and then postincubated to allow inducible stress functions to develop. The magnitude of the DNA damage response was monitored with respect to DNA-PK phosphorylation of a p53 peptide, defence protein levels (Ku, DNA-PK catalytic subunit, ATM, p21 and p53) and flow cytometric determination of cell cycle phases and apoptosis. Results. B cells from SS patients, compared with healthy controls, displayed enhancement of two stress functions in undamaged cells: DNA-PK kinase activity and apoptosis. In addition, SS showed enhanced cell cycle arrest in gamma-irradiated cells. Conclusions. Strong kinase activity of DNA-PK, functioning not only in a DNA damage response but also in immunoglobulin gene rearrangement, may be an important component of the heightened stress response displayed by SS cells. In combination with recent reports, our data indicate that constitutional hyper-reactivity to danger signals is a basic pathogenetic factor in SS.
DOI http://dx.doi.org/10.1093/rheumatology/keh267 (link to publisher's fulltext)
Host/Issue Rheumatology
Volume 43
ISSN 1462-0324
Pages 1109-1115
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/3003 Permalink to this page

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