Coarse-grained modeling of Proline Rich Protein 1 in Bulk Solution and Adsorbed to a Negatively Charged Surface

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Coarse-grained modeling of Proline Rich Protein 1 in Bulk Solution and Adsorbed to a Negatively Charged Surface

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Publication Article, peer reviewed scientific
Title Coarse-grained modeling of Proline Rich Protein 1 in Bulk Solution and Adsorbed to a Negatively Charged Surface
Author(s) Skepö, Marie ; Arnebrant, Thomas ; Linse, Per
Date 2006
English abstract
Structural properties of the acidic proline rich protein PRP-1 of salivary origin in bulk solution and adsorbed onto a negatively charged surface have been studied by Monte Carlo simulations. A simple model system with focus on electrostatic interactions and short-ranged attractions among the uncharged amino acids has been used. In addition to PRP-1, some mutants were considered to assess the role of the interactions in the systems. Contrary to polyelectrolytes, the protein has a compact structure in salt-free bulk solutions, whereas at high salt concentration the protein becomes more extended. The protein adsorbs to a negatively charged surface, although its net charge is negative. The adsorbed protein displays an extended structure, which becomes more compact upon addition of salt. Hence, the conformational response upon salt addition in the adsorbed state is the opposite as compared to that in bulk solution. The conformational behaviour of PRP-1 in bulk solution and at charged surfaces as well as its propensity to adsorb to surfaces with the same net charge are rationalized by the block polyampholytic character of the protein. The presence of a triad of positively charged amino acids in the C-terminal was found important for the adsorption of the protein.
Host/Issue Journal of Physical Chemistry B
Language eng (iso)
Subject(s) Proteins
simulations
MonteCarlo
saliva
surfaces
bulk
Sciences
Handle http://hdl.handle.net/2043/3213 (link to this page)

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