Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide

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Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide

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Publication Article, peer reviewed scientific
Title Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide
Author(s) Ruzgas, Tautgirdas ; Shleev, Sergey
Date 2006
English abstract
A novel biosensor for superoxide radical (O2-) detection based on Pseudomonas aeruginosa azurin immobilized on gold electrode was designed. The rate constant of azurin reduction by O2- was found to be 105M−1 s−1 in solution and five times lower, i.e., 0.2x105M−1 s−1, for azurin coupled to gold by 3,3-dithiobis(sulfosuccinimidylpropionate) (DTSSP). The electron transfer rate between the protein and the electrode ranged from 2 to 6 s−1. The sensitivity of this biosensor to O2− was 6.8x102Am−2M−1. The response to the interference substances, such as uric acid, H2O2, and dimethylsulfoxide was negligible below 10 M. The electrode was applied in three O2− generating systems: (i) xanthine oxidase (XOD), (ii) potassium superoxide (KO2), and (iii) stimulated neutrophil granulocytes. The latter was compared with luminol-amplified chemiluminescence. The biosensor responded to O2− in all three environments, and the signals were antagonized by superoxide dismutase.
DOI http://dx.doi.org/10.1016/j.bios.2005.12.017 (link to publisher's fulltext)
Host/Issue Biosensors & Bioelectronics;2
Volume 22
Pages 213-219
Language swe (iso)
Subject(s) Azurin
Superoxide
Medicine
Research Subject Categories::NATURAL SCIENCES
Research Subject Categories::NATURAL SCIENCES::Chemistry
Handle http://hdl.handle.net/2043/3289 Permalink to this page

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