Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation

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Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation

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Publication Article, peer reviewed scientific
Title Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation
Author(s) Svendsen, Ida ; Lindh, Liselott ; Arnebrant, Thomas
Date 2007
English abstract
The acquired pellicle is the thin salivary film that covers all oral surfaces, formed by selective adsorption of primarily salivary proteins. Several cationic proteins, such as lactoferrin, lactoperoxidase, lysozyme and histatin 5 have been identified in the pellicle. This study focused on the adsorption of lactoperoxidase and histatin 5, to investigate their possible importance in the initial salivary film formation. The adsorption was investigated by means of in situ ellipsometry, to both pure (hydrophilic) and methylated (hydrophobized) silica substrates, at concentrations relevant in saliva. The adsorption was investigated in terms of surface area per molecule and influence of surface wettability. Mass transport controlled adsorption in relation to the initial adsorption from human whole saliva and glandular saliva was also investigated. Results showed that lactoperoxidase adsorbed in larger amounts on pure silica compared to methylated surfaces. Histatin 5 adsorbed to the same extent to the two types of surfaces, but to a lesser extent compared to lactoperoxidase. The mass transport calculated adsorption rates of lactoperoxidase and histatin 5 showed that histatin 5 might potentially have a significant role in the initial adsorption from saliva, whereas lactoperoxidase may also adsorb but is not a dominating component.
DOI http://dx.doi.org/10.1524./zpch.2007.221.1.65 (link to publisher's fulltext)
Publisher Oldenbourg Wissenschaftsverlag, München
Host/Issue Zeitschrift fur physikalische chemie
Volume 221
ISSN 0942-9352
Pages 1-9
Language eng (iso)
Subject(s) Lactoperoxidase
Histatin 5
Ellipsometry
Pellicle
surface chemistry
biological interfaces
Sciences
Research Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Surface and colloid chemistry Research Subject
Handle http://hdl.handle.net/2043/3382 (link to this page)
Link http://www.atypon-link.com/OLD/toc/zpch/221/1 (external link to related web page)

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