The aim of this study was to compile scientific knowledge of health personnel’s attitudes towards hand hygiene. The result is based on 10 scientific articles. The result shows that health personnel have a positive attitude toward hand hygiene when contact with patients or exposure of body fluids. On the other hand, negative attitude are shown when using gloves, believes that hand hygiene causes skin irritation or when hand disinfection is not easily accessible. Personnel’s knowledge and their superior’s opinions about hand hygiene have an effect on the personnel’s attitude. The summary is that more knowledge among the personnel is needed to change their attitudes and behaviour of hand hygiene. More research about health personnel’s attitudes towards hand hygiene are even needed in a more specific way where different professions attitudes are investigated, so that education can adjust to their particular needs.