Concentration dependence of adsorption from human whole resting saliva at solid/liquid interfaces: An ellipsometric study

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Concentration dependence of adsorption from human whole resting saliva at solid/liquid interfaces: An ellipsometric study

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Publication Article, peer reviewed scientific
Title Concentration dependence of adsorption from human whole resting saliva at solid/liquid interfaces: An ellipsometric study
Author(s) Lindh, Liselott ; Arnebrant, Thomas ; Isberg, Per-Erik ; Glantz, Per-Olof
Date 1999
English abstract
The rates and plateau values of adsorption at the solid/ liquid interface from human whole resting saliva from three male donors were studied in vitro. The influence of saliva concentration, the total protein content and the wettability of the exposed solids was investigated. Ellipsometry was used to study the rate and plateau values of the adsorption process in situ. The solid surfaces used were hydrophilic, negatively charged, silica and hydrophobic methylated silica. Salivary films were formed by adsorption from solutions of 0.05%, 0.10%, 0.20%, 0.50%, 1.0% and 2.0% (v/v) saliva in phosphate buffered saline. The results revealed that adsorption onto both types of surfaces was a rapid and, as expected, a concentration dependent process. The adsorbed amounts were in the range of monomolecular layers and were found to be larger onto the hydrophobic than the hydrophilic surfaces (p≤0.0062). A strong concentration dependence was denoted in all cases (p< 0.0001). Inter-individual differences were found not to be significant (0.0636 ≤ p≤ 0.8508) at any of the saliva concentrations. However, an interaction was shown (p < 0.0001) between the protein concentrations and the donors, indicating qualitative differences in protein content and surface activity.
DOI http://dx.doi.org/10.1080/08927019909378410 (link to publisher's fulltext)
Host/Issue Biofouling;3
Volume 14
ISSN 0892-7014
Pages 189-196
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/4064 (link to this page)

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