On the adsorption of human proline-rich proteins (PRP-1 and PRP-3) and statherin at solid/liquid interfaces

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On the adsorption of human proline-rich proteins (PRP-1 and PRP-3) and statherin at solid/liquid interfaces

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Publication Article, peer reviewed scientific
Title On the adsorption of human proline-rich proteins (PRP-1 and PRP-3) and statherin at solid/liquid interfaces
Author(s) Lindh, Liselott ; Glantz, Per-Olof ; Strömberg, Nicklas ; Arnebrant, Thomas
Date 2002
English abstract
The objective of the present study was to investigate the adsorption of PRP-1, PRP-3 and statherin to solid surfaces in terms of dependence on concentration, the presence of electrolyte and surface wettability. Time resolved in situ ellipsometry was used to determine the adsorbed amounts and adsorption rates of pure PRP-1, PRP-3 and statherin onto pure (hydrophilic) and methylated (hydrophobized) silica surfaces. The initial film build-up was fast and plateaus were reached within 10 min at all concentrations for both types of surfaces and all proteins. The observed adsorption and calculated diffusion rates of PRP-1, PRP-3 and statherin, respectively, indicated that the initial adsorption was mass transport controlled at low concentrations. At hydrophobic surfaces, isotherm shapes and adsorbed amounts were similar for PRP-1 and PRP-3, while statherin adsorbed to a higher extent. At hydrophilic surfaces only PRP-1 adsorbed substantially, while for PRP-3 and statherin adsorbed amounts were low. The presence of Ca 2+ ions in the phosphate buffer solution increased the adsorption of statherin and PRP-3 on hydrophobic surfaces, while PRP-1 was unaffected. On hydrophilic surfaces, all three proteins adsorbed in higher amounts in NaCl, compared to CaCl 2 at similar ionic strength. It is concluded that acidic PRPs (PRP-1 and PRP-3) and statherin readily form films on a variety of materials and solution conditions, showing that their functions may be fulfilled under a wide range of conditions.
DOI http://dx.doi.org/10.1080/08927010290000110 (link to publisher's fulltext)
Host/Issue Biofouling;2
Volume 18
ISSN 0892-7014
Pages 87-94
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/4074 (link to this page)

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