The salivary mucin MUC5B and lactoperoxidase can be used for layer-by-layer film formation

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The salivary mucin MUC5B and lactoperoxidase can be used for layer-by-layer film formation

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Publication Article, peer reviewed scientific
Title The salivary mucin MUC5B and lactoperoxidase can be used for layer-by-layer film formation
Author(s) Lindh, Liselott ; Svendsen, Ida ; Svensson, Olof ; Cardenas, Marité ; Arnebrant, Thomas
Date 2007
English abstract
Abstract In situ ellipsometry was used to study layer-by-layer film formation on hydrophilic and hydrophobized silica surfaces by alternating sequential adsorption of human mucin MUC5B and cationic proteins lysozyme, lactoferrin, lactoperoxidase or histatin 5, respectively. The stability of the multilayers was investigated by addition of sodium dodecyl sulfate solution (SDS). Atomic force microscopy was employed to investigate morphological structures on the surfaces during the layer-by-layer film build-up. It was clearly shown that, on both hydrophilic and hydrophobized silica, only MUC5B and lactoperoxidase showed the ability for multilayer formation, resulting in an approximately linear increase in adsorbed amount and film thickness with each deposition cycle. The net increase in amounts per cycle was larger on the hydrophilic silica. Further, MUC5B needs to be adsorbed first on the hydrophilic substrates to obtain this fast build-up behavior. Generally, addition of SDS solution showed that a large fraction of the adsorbed film could be desorbed. However, films on the hydrophobized silica were more resistant to surfactant elution. In conclusion, MUC5B–cationic protein multilayers can be formed on hydrophilic and hydrophobized silica, depending on the choice of the cationic protein as well as in which order the build-up is started on hydrophilic silica. Additionally, SDS disrupts the layer-by-layer film formed by MUC5B and lactoperoxidase.
DOI http://dx.doi.org/10.1016/j.jcis.2007.01.086 (link to publisher's fulltext)
Host/Issue Journal of Colloid Interface Science;1
Volume 310
Pages 74-82
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Surface and colloid chemistry
Research Subject Categories::TECHNOLOGY::Bioengineering::Structural biochemistry
Handle http://hdl.handle.net/2043/4474 (link to this page)

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