Solubilization of sphingomyelin vesicles by addition of a bile salt

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Solubilization of sphingomyelin vesicles by addition of a bile salt

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Publication Article, peer reviewed scientific
Title Solubilization of sphingomyelin vesicles by addition of a bile salt
Author(s) Cardenas, Marité ; Arnebrant, Thomas ; Schillén, Karin ; Alfredsson, Viveka ; Duan, Rui-DOng ; Nyberg, Lena
Date 2007
English abstract
The interactions of the bile salt sodium taurocholate (TC) in 50 mM Trizma–HCl buffer and 150 mM NaCl (pH 9) at 37 °C with membranes composed of sphingomyelin (SM) were studied by dynamic light scattering, cryogenic transmission electron microscopy (cryo-TEM) and turbidity measurements. Small unilamellar SM vesicles were prepared by extrusion. Below the CMC of TC, taurocholate addition leads to vesicle growth due to incorporation of the taurocholate molecules into the vesicle bilayer. At around half the CMC of the bile salt, the SM vesicles are transformed into SM/TC mixed worm-like micelles, which are visualized by cryo-TEM for the first time. Further increase in the taurocholate concentration leads to the rupture of these structures into small spherical micelles. Interestingly, large non-spherical micelles were also identified for pure taurocholate solutions. Similar threadlike structures have been reported earlier for the bile salt sodium taurodeoxycholate [Rich, A., Blow, D., 1958. Nature 182, 1777; Blow, D.M., Rich, A., 1960. J. Am. Chem. Soc. 82, 3566–3571; Galantini, L., Giglio, E., La Mesa, C., Viorel-Pavel, N., Punzo, F., 2002. Langmuir 18, 2812] and for mixtures of taurocholate and phosphatidylcholate [Ulmius, J., Lindblom, G., Wennerström, H., Johansson, L.B.-Å., Fontel, K., Söderman, O., Ardvisson, G., 1982. Biochemistry 21, 1553; Hjelm, R.P., Thiyagarajan, P., Alkan-Onyuksel, H., 1992. J. Phys. Chem. 96, 8653] as determined by various scattering methods.
DOI (link to publisher's fulltext)
Host/Issue Chemistry and Physics of Lipids
Language eng (iso)
Subject(s) Sciences
Research Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Surface and colloid chemistry
Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology
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