Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells

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Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells

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Publication Article, peer reviewed scientific
Title Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells
Author(s) Jönsson, Daniel ; Nilsson, Jenny ; Odenlund, Malin ; Bratthall, Gunilla ; Broman, Jonas ; Ekblad, Eva ; Lydrup, Marie-Louise ; Nilsson, Bengt-Olof
Date 2007
English abstract
Objective: Periodontal ligament (PDL) cells express oestrogen receptor b (ERb) protein, but cellular functions regulated by ERb in these cells have not been identified. In this study we determine if ERb is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. Design: Subcellular distribution of ERb was determined by confocal microscopy of cells costained with ERb antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17b-oestradiol. Results: ERb immunoreactivity was observed both in the nuclei and the cytoplasm. Mito- Tracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERb and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERb was confirmed by immunogold electron microscopy. Cells treated with or without 17b-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17b-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17b-oestradiol and the ER blocker ICI 182780 (10 mM) had no effect. Conclusion: This study demonstrates mitochondrial localisation of ERb and oestrogeninduced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERb influences mitochondrial function and PDL cell energy metabolism.
DOI http://dx.doi.org/10.1016/j.archoralbio.2006.12.009 (link to publisher's fulltext)
Host/Issue Archives of Oral Biology;7
Volume 52
ISSN 0003-9969
Pages 669-76
Language eng (iso)
Subject(s) Periodontal ligament cells
estrogen receptor
Mitochondria
Medicine
Research Subject Categories::ODONTOLOGY::Periodontology
Research Subject Categories::MEDICINE::Dermatology and venerology,clinical genetics, internal medicine::Internal medicine::Endocrinology
Handle http://hdl.handle.net/2043/4627 (link to this page)

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