Acid Tolerance of Biofilm Cells of Streptococcus mutans

DSpace Repository

Acid Tolerance of Biofilm Cells of Streptococcus mutans

Show full item record

Files for download

Find Full text There are no files associated with this item.


Simple item record

Publication Article, peer reviewed scientific
Title Acid Tolerance of Biofilm Cells of Streptococcus mutans
Author(s) Neilands, Jessica ; Svensäter, Gunnel
Date 2007
English abstract
Streptococcus mutans, a member of the dental plaque community, has been shown to be involved in the carious process. Cells of S. mutans induce an acid tolerance response (ATR) when exposed to sublethal pH values that enhances their survival at a lower pH. Mature biofilm cells are more resistant to acid stress than planktonic cells. We were interested in studying the acid tolerance and ATR-inducing ability of newly adhered biofilm cells of S. mutans. All experiments were carried out using flow-cell systems, with acid tolerance tested by exposing 3-h biofilm cells to pH 3.0 for 2 h and counting the number of survivors by plating on blood agar. Acid adaptability experiments were conducted by exposing biofilm cells to pH 5.5 for 3 h and then lowering the pH to 3.5 for 30 min. The viability of the cells was assessed by staining the cells with LIVE/DEAD BacLight viability stain. Three-hour biofilm cells of three different strains of S. mutans were between 820- and 70,000-fold more acid tolerant than corresponding planktonic cells. These strains also induced an ATR that enhanced the viability at pH 3.5. The presence of fluoride (0.5 M) inhibited the induction of an ATR, with 77% fewer viable cells at pH 3.5 as a consequence. Our data suggest that adhesion to a surface is an important step in the development of acid tolerance in biofilm cells and that different strains of S. mutans possess different degrees of acid tolerance and ability to induce an ATR.
DOI (link to publisher's fulltext)
Publisher American Society for Microbiology
Host/Issue Applied and Environmental Microbiology;17
Volume 73
ISSN 0099-2240
Pages 5633-5638
Language eng (iso)
Subject(s) Fluoride
Streptococcus mutans
Acid tolerance
Research Subject Categories::ODONTOLOGY::Oral microbiology
Handle (link to this page)

This item appears in the following Collection(s)

Show full item record



My Account