Studies on the exchange of early pellicle proteins by mucin and whole

DSpace Repository

Studies on the exchange of early pellicle proteins by mucin and whole

Show full item record

Files for download

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title Studies on the exchange of early pellicle proteins by mucin and whole
Author(s) Svendsen, Ida ; Lindh, Liselott ; Elofsson, Ulla ; Arnebrant, Thomas
Date 2008
English abstract
Adsorption of small pellicle proteins statherin or proline-rich protein 1 (PRP1) respectively, and subsequent adsorption of human whole saliva (HWS) or salivary mucin MUC5B respectively, was studied using ellipsometry and total internal reflectance fluorescence. Differences in elution (using sodium dodecyl sulphate (SDS) solutions) between mixed and single protein films were also investigated. On both hydrophilic and hydrophobized surfaces HWS and MUC5B were found to adsorb to pre-adsorbed layers of statherin and PRP1 respectively. Statherin adsorption on both substrate types showed no or minor exchange by HWS or MUC5B and no change in SDS elution between mixed and single protein films. Small amounts of PRP1 were exchanged by HWS on both surface types and the SDS elutable fractions were similar or larger for mixed films compared to single protein films. PRP1 and MUC5B in sequence showed minor exchange of PRP1 on hydrophilic surfaces, while no exchange could be established on hydrophobized substrates. SDS elutable fractions decreased for PRP1 and MUC5B mixed films compared to single protein films. In conclusion, minor amounts of statherin and PRP1 are exchanged during the time course of the experiments, which indicates that these proteins may to a large extent remain incorporated in the pellicle.
DOI http://dx.doi.org/10.1016/j.jcis.2008.01.041 (link to publisher's fulltext)
Publisher Elsevier
Host/Issue Journal of Colloid and Interface Science;1
Volume 321
ISSN 0021-9797
Pages 52-59
Language eng (iso)
Subject(s) Total internal reflection fluorescence
ellipsometry
hydrophilic surfaces
hydrophobized surfaces
FITC
SDS
MUC5B
proline-rich proteins
statherin
Sciences
Research Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Surface and colloid chemistry
Research Subject Categories::ODONTOLOGY::Other odontology
Handle http://hdl.handle.net/2043/6131 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics