Validation of mechanically-assisted sodium dodecyl-sulphate elution as a technique to remove pellicle protein components from human enamel

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Validation of mechanically-assisted sodium dodecyl-sulphate elution as a technique to remove pellicle protein components from human enamel

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Publication Article, peer reviewed scientific
Title Validation of mechanically-assisted sodium dodecyl-sulphate elution as a technique to remove pellicle protein components from human enamel
Author(s) Svendsen, Ida ; Arnebrant, Thomas ; Lindh, Liselott
Date 2008
English abstract
The salivary film, denoted the pellicle, formed on oral surfaces is of great importance for oral health and comfort. The present study describes mechanically-assisted sodium dodecyl sulphate (SDS) elution of the in vivo pellicle formed on human enamel and visualisation of the desorbed pellicle proteins using two-dimensional gel electrophoresis (2-DE). To verify this removal of the pellicle, a combined mechanical and surfactant procedure was additionally performed on an in vitro pellicle formed on human enamel, and the effectiveness was validated by mechanical removal in combination with HCl. As indicated by protein quantitation and one dimensional gel electrophoresis, rubbing with polyamide fibre pellets soaked in a 0.5% SDS solution was optimal for completely removing the adsorbed proteins from the enamel surface, and yet provided separation of the proteins by 2-DE to enable identification in future studies.
Publisher informaworld
Host/Issue Biofouling
Volume 24
Pages 227-233
Language eng (iso)
Subject(s) saliva
gel electrophoresis
surfactant
validation studies
enamel surface
Sciences
Research Subject Categories::NATURAL SCIENCES::Chemistry::Physical chemistry::Surface and colloid chemistry
Research Subject Categories::ODONTOLOGY::Biochemistry
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry
Handle http://hdl.handle.net/2043/6132 (link to this page)

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