Protein expression and cellular localization in two prognostic subgroups of diffuse large B-cell lymphoma: Higher expression of ZAP70 and PKC-beta II in the non-germinal center group and poor survival in patients deficient in nuclear PTEN

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Protein expression and cellular localization in two prognostic subgroups of diffuse large B-cell lymphoma: Higher expression of ZAP70 and PKC-beta II in the non-germinal center group and poor survival in patients deficient in nuclear PTEN

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Publication Article, peer reviewed scientific
Title Protein expression and cellular localization in two prognostic subgroups of diffuse large B-cell lymphoma: Higher expression of ZAP70 and PKC-beta II in the non-germinal center group and poor survival in patients deficient in nuclear PTEN
Author(s) Gjörloff Wingren, Anette ; Fridberg, Marie ; Servin, Anna ; Anagnostaki, Lola ; Linderoth, Johan ; Berglund, Mattias ; Söderberg, Ola ; Enblad, Gunilla ; Rosén, Anders ; Mustelin, Tomas ; Jerkeman, Mats ; Persson L, Jenny ; Fridberg, Marie ; Servin, Anna ; Anagnostaki, Lola ; Linderoth, Johan ; Berglund, Mattias ; Söderberg, Ola ; Enblad, Gunilla ; Rosén, Anders ; Mustelin, Tomas ; Jerkeman, Mats ; Persson, Jenny
Date 2007-10
English abstract
Patients diagnosed with diffuse large B-cell lymphoma (DLBCL) show varying responses to conventional therapy, and this might be contributed to the differentiation stage of the tumor B-cells. The aim of the current study was to evaluate a panel of kinases (ZAP70, PKC-beta I and II and phosphorylated PKB/Akt) and phosphatases (PTEN, SHP1 and SHP2) known to be frequently deregulated in lymphoid malignancies. De novo DLBCL cases were divided into two subgroups, the germinal center (GC) group (14/28) and the non-germinal center (non-GC) or activated B-cell (ABC) group (14/28). ZAP70 and PKC-beta II were expressed in a significantly higher percentage of tumor cells in the clinically more aggressive non-GC group compared with the prognostically favourable GC group. Also, the subcellular localization of PKC-beta I and II differed in DLBCL cells, with the PKC-beta I isoform being expressed in both the cytoplasm and nucleus, while PKC-beta II was found exclusively in the cytoplasm. Loss of nuclear PTEN correlated with poor survival in cases from both subgroups. In addition, five cell lines of DLBCL origin were analyzed for protein expression and for mRNA levels of PTEN and SHP1. For the first time, we show that ZAP70 is expressed in a higher percentage of tumor cells in the aggressive non-GC subgroup of DLBCL and that PKC-beta I and II are differently distributed in the two prognostic subgroups of de novo DLBCL.
DOI http://dx.doi.org/10.1080/10428190701636443 (link to publisher's fulltext)
Publisher InformaWorld
Host/Issue Leukemia Lymphoma;11
Volume 48
ISSN 1042-8194
Pages 2221-32
Language eng (iso)
Subject(s) lymphoma
immunohistochemistry
Medicine
Research Subject Categories::MEDICINE::Morphology, cell biology, pathology::Cell biology::Medical cell biology
Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Immunology::Tumour immunology
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/6408 (link to this page)

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