SENSITIVE DETECTION OF HYDROPHOBIC ANTIGENS USING A NOVEL LIPID-AGGREGATE BASED ELISA

DSpace Repository

SENSITIVE DETECTION OF HYDROPHOBIC ANTIGENS USING A NOVEL LIPID-AGGREGATE BASED ELISA

Show full item record

Files for download

Find Full text There are no files associated with this item..

Facebook

Simple item record

Publication Article, peer reviewed scientific
Title SENSITIVE DETECTION OF HYDROPHOBIC ANTIGENS USING A NOVEL LIPID-AGGREGATE BASED ELISA
Author(s) Ketelsen, Anna ; Eriksson, Håkan
Date 2008-10-23
English abstract
Antibodies against hydrophobic antigens are common in several autoimmune diseases. However, detection of such antibodies by standard immune-assays, such as ELISA, is problematic, in part because of the problems with coating hydrophobic molecules onto polystyrene multi-well plates. We describe a novel method of stably associating hydrophobic antigens to ELISA plates. By mixing the antigen with a hydrophobic molecule containing a hydrophilic anchor, we generate mixed lipid aggregates that can attach to ELISA plates, and are resistant to detergent wash. Using the ganglioside GM-1 and phosphatidylethanolamine conjugated to the hapten DNP (dinitrophenyl) as model antigens, we show that hydrophobic antigens incorporated into mixed lipid aggregates expose their antigenic determinants in a correct configuration. The detection limit of both GM-1 and DNP-PE was considerably improved compared to when these antigens were coated on ELISA plates using organic solvents. Furthermore GM-1 incorporated into mixed lipid aggregates can be detected by specific antibodies in patient serum. The method of incorporating hydrophobic antigens into mixed lipid aggregates for stable association to ELISA plates can presumably be applied to a vast array of hydrophobic antigens, and may well be developed into a large scale screening system for serum reactivity towards different hydrophobic antigens.
DOI http://dx.doi.org/10.1016/j.jim.2008.09.012 (link to publisher's fulltext)
Host/Issue Journal of Immunological Methods;2008
Language eng (iso)
Subject(s) ELISA
ganglioside
hydrophobic antigen
Humanities/Social Sciences
Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases
Research Subject Categories::NATURAL SCIENCES::Chemistry
Handle http://hdl.handle.net/2043/6633 (link to this page)

This item appears in the following Collection(s)

Show full item record

Search


Browse

My Account

Statistics