DSP and Collagen I Expression After Pulp Capping in Humans

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DSP and Collagen I Expression After Pulp Capping in Humans

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Publication Conference Paper, peer reviewed
Title DSP and Collagen I Expression After Pulp Capping in Humans
Author(s) Fransson, Helena ; Petersson, Kerstin ; Davies, Julia
Date 2008
English abstract
Formation of hard tissue following treatment of pulp exposures has been observed in both clinical and experimental studies. However it is not known if such tissue has the functions of primary dentin which are likely to be important in protecting the pulp from the oral environment. Objectives: To study the expression of two relatively specific markers for dentin; dentin sialoprotein (DSP) and type 1 collagen (Col I) in human teeth experimentally pulp capped either with Emdogain®Gel (Biora AB, Malmö, Sweden) - a derivative of enamel matrix (EMD) in a propylene glycol alginate vehicle, or with calcium hydroxide. Methods: Nine pairs of contra-lateral premolars scheduled for extraction on orthodontic grounds were experimentally pulp amputated and capped with EMDgel or calcium hydroxide. After 12 weeks the teeth were extracted, prepared for light microscopic examination and stained using affinity-purified rabbit anti-Col I and anti-DSP polyclonal antibodies. Results: In the EMDgel treated teeth, new tissue partly filled the space initially occupied by the gel and hard tissue was formed alongside exposed dentin surfaces and in patches in the adjacent pulp tissue. In the calcium hydroxide treated teeth, the new hard tissue was formed as a bridge. DSP staining was seen in the newly formed hard tissue and in the cells lining it in both groups but was more marked in the EMDgel treated teeth. Col I staining was seen in the newly formed hard tissue in both groups. Conclusion: The expression of DSP and Col I suggests that the new hard tissue formed after pulp capping with EMDgel or calcium hydroxide is dentin. Supported by Biora AB, Malmö, Sweden, the European Society of Endodontology and the Swedish Dental Society.
Publisher IADR General session, Toronto, ON, Canada 2-5 july 2008
Language eng (iso)
Subject(s) Humanities/Social Sciences
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/6972 (link to this page)

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