Protein Profile of Pepsin-digested Carious and Sound Human Den-tine

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Protein Profile of Pepsin-digested Carious and Sound Human Den-tine

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Publication Article, peer reviewed scientific
Title Protein Profile of Pepsin-digested Carious and Sound Human Den-tine
Author(s) Tonami, Ken- Ichi ; Ericson, Dan
Date 2005
English abstract
The purpose of this study was to describe the protein profile of pepsin-digested carious and sound dentine using sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE). Carious and sound den-tine powder was decalcified using 10% EDTA at pH 7.4 for 48 h. The decalcified pellet was digested using pepsin at pH 2 under sequenced conditions: at 4 degrees C for 24 h, a further 24 h at 23 degrees C, and finally for 24 h at 37 degrees C. After every step, the soluble frac-tion was separated by centrifugation and analyzed in 15% SDS-PAGE. Two bands at 56 and 62 kDa could be observed in carious dentine di-gests and were considered specific carious bands. Similar bands could be observed in sound dentine samples, but only after pepsin digestion at higher temperatures (23 degrees C and 37 degrees C). Pepsin digests non-helical collagen and the triple helix structure of collagen is lost when the temperature rises. The bands at 56 and 62 kDa in sound dentine specimens thus represent pepsin-cleaved collagen. There is a possibility that the specific carious bands in carious dentine represent collagen decomposed in a manner similar to the way pepsin digests na-tive dentine collagen at 23 degrees C and 37 degrees C.
DOI (link to publisher's fulltext)
Host/Issue Acta Odontologica Scandinavica;1
Volume 63
ISSN 0001-6357
Pages 17-20
Language eng (iso)
Subject(s) Medicine
Research Subject Categories::ODONTOLOGY
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