Salivary IgA reactions to cell-surface antigens of oral streptococci.

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Publication Article, peer reviewed scientific
Title Salivary IgA reactions to cell-surface antigens of oral streptococci.
Author(s) Wallengren, Marie-Louise ; Ericson, Dan ; Hamberg, Kristina
Date 2004
English abstract
BACKGROUND: In the immunoblot technique, using whole bacteria cell extracts as antigens, both intra- and extracellular antigens are de-tected, which gives a large number of immunoglobulin A (IgA) reac-tions (immunoblot bands) when incubated with saliva. It is important to distinguish which immunoblot bands represent bacterial cell-surface antigens, since these antigens could be involved in adhesion mecha-nisms and be available for blocking in vivo. METHODS: Bacterial ex-tracts of Streptococcus mutans, Streptococcus sobrinus, Streptococcus parasanguis and the streptococcal antigen I/II were separated using SDS-PAGE. The antigens were detected with saliva in Western blot. Untreated saliva and saliva in which cell-surface reactive IgA had been absorbed with whole bacteria cells were analyzed. RESULTS: Ap-proximately half the number of the bands were absent for saliva ab-sorbed with homologous cells, compared to untreated saliva. The ab-sorption pattern was almost identical for S. mutans and S. sobrinus but not for S. parasanguis. Salivary IgA reactive against streptococcal antigen I/II was absorbed by S. mutans cells, to a lesser extent by S. sobrinus cells, and not at all by S. parasanguis cells. CONCLUSION: It is likely that the bands that were absent after absorption represented cell-surface antigens. For S. mutans and S. sobrinus, these bands were probably the streptococcal antigen I/II. Copyright Blackwell Munks-gaard, 2004.
DOI (link to publisher's fulltext)
Publisher Blackwell Publishing
Host/Issue Oral Microbiology and Immunology;3
Volume 19
ISSN 0902-0055
Pages 188-95
Language eng (iso)
Subject(s) cell-surface antigen
Oral streptococci
salivary IgA
Western blot
Research Subject Categories::ODONTOLOGY
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