Direct electron transfer reactions between human ceruloplasmin and electrodes

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Direct electron transfer reactions between human ceruloplasmin and electrodes

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Publication Article, peer reviewed scientific
Title Direct electron transfer reactions between human ceruloplasmin and electrodes
Author(s) Shleev, Sergey
Date 2009
English abstract
In an effort to find conditions favoring bioelectrocatalytic redn. of oxygen by surface-immobilized human ceruloplasmin (Cp), direct electron transfer (DET) reactions between Cp and an extended range of surfaces were considered. Exploiting advances in surface nanotechnol., bare and carbon-nanotube-modified spectrog. graphite electrodes as well as bare, thiol- and gold-nanoparticle-modified gold electrodes were considered, and ellipsometry provided clues as to the amt. and form of adsorbed Cp. DET was studied under different conditions by cyclic voltammetry and chronoamperometry. Two Faradaic processes with midpoint potentials of about 400 mV and 700 mV vs. NHE, corresponding to the redox transformation of copper sites of Cp, were clearly obsd. In spite of the significant amt. of Cp adsorbed on the electrode surfaces, as well as the quite fast DET reactions between the redox enzyme and electrodes, bioelectrocatalytic redn. of oxygen by immobilized Cp was never registered. The bioelectrocatalytic inertness of this complex multi-functional redox enzyme interacting with a variety of surfaces might be assocd. with a very complex mechanism of intramol. electron transfer involving a kinetic trapping behavior.
DOI http://dx.doi.org/10.1016/j.bioelechem.2009.05.012 (link to publisher's fulltext)
Publisher Elsevier B.V.
Host/Issue Bioelectrochemistry;1-2
Volume 76
ISSN 1567-5394
Pages 34-41
Language eng (iso)
Subject(s) ceruloplasmin
copper sites
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/8559 (link to this page)

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