Bioelectrochemical studies of azurin and laccase confined in three-dimensional chips based on gold-modified nano-/microstructured silicon

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Bioelectrochemical studies of azurin and laccase confined in three-dimensional chips based on gold-modified nano-/microstructured silicon

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Publication Article, peer reviewed scientific
Title Bioelectrochemical studies of azurin and laccase confined in three-dimensional chips based on gold-modified nano-/microstructured silicon
Author(s) Ressine, Anton ; Vaz-Dominguez, Cristina ; Fernandez, Victor M ; De Lacey, Antonio L ; Laurell, Thomas ; Ruzgas, Tautgirdas ; Shleev, Sergey
Date 2010
English abstract
Double-sided three-dimensional porous silicon chips, 6 mm × 6 mm, covered with a 40 nm gold (nano)layer, were fabricated from a porous silicon wafer. Scanning electron microscopy along with electrochemical characterisation showed sample conductivity, mechanical stability, and high surface area of the thus fabricated devices, viz. 10 times higher electrochemically active surface area compared to the geometric area. The three-dimensional gold coated silicon chips were further modified with thiol layers, followed by immobilisation of a simple copper-containing redox protein, azurin, or a complex multicopper redox enzyme, laccase. The bioelectrochemical studies showed very high surface concentrations of azurin and laccase, i.e. close to the theoretical monolayer coverage. However, direct electron transfer reactions between the biomolecules and gold surfaces were observed only for a small percentage of the immobilised redox protein and enzyme, respectively. Thus, highly efficient oxygen-bioelectroreduction on laccase-modified 3D thiol-gold-porous silicon chips (as compared to planar laccase-modified gold electrodes, 42 μA/cm2 vs. 7 μA/cm2, respectively) was obtained only in the presence of an efficient soluble redox mediator.
DOI http://dx.doi.org/10.1016/j.bios.2009.09.014 (link to publisher's fulltext)
Publisher Science direct
Host/Issue Biosensors and Bioelectronics;5
Volume 15
ISSN 0956-5663
Pages 1001-1007
Language eng (iso)
Subject(s) Porous silicon
Three-dimensional biochip
Biosensor
Biocathode
Azurin
Laccase
Medicine
Research Subject Categories::MEDICINE::Chemistry::Biochemistry
Handle http://hdl.handle.net/2043/9977 (link to this page)

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